Author: Chen, Huanzhu; Weng, Huilan; Lin, Meirui; He, Ping; Li, Yazhen; Xie, Qingdong; Ke, Changwen; Jiao, Xiaoyang
Title: The Clinical Significance of FilmArray Respiratory Panel in Diagnosing Community-Acquired Pneumonia Document date: 2017_9_6
ID: 1awz5712_7
Snippet: The specimens collected from nasal mucus were stored in swab storage solution (COPAN, Italy) for multiplex PCR and FilmArray RP assay. The nucleic acid was extracted by using beads viral DNA/RNA extraction kit (TIANLONG, China) and NP968 nucleic acid extraction instrument (TIANLONG, China). We tested 14 viruses, including influenza viruses types A and B (Flu A and Flu B), parainfluenza viruses 1 to 4 (PIV1-4), respiratory syncytial virus (RSV), c.....
Document: The specimens collected from nasal mucus were stored in swab storage solution (COPAN, Italy) for multiplex PCR and FilmArray RP assay. The nucleic acid was extracted by using beads viral DNA/RNA extraction kit (TIANLONG, China) and NP968 nucleic acid extraction instrument (TIANLONG, China). We tested 14 viruses, including influenza viruses types A and B (Flu A and Flu B), parainfluenza viruses 1 to 4 (PIV1-4), respiratory syncytial virus (RSV), coronaviruses 229E, OC43, HKU1, and NL63, human metapneumovirus (MPV), human rhinovirus (HRV), and adenovirus (ADV), were detected using a single-tube TaqMan5 real-time reverse transcription PCR strategy (AgPath-ID6 One-Step RT-PCR Kit Applied Biosystems, USA). PCR amplification was performed using a 7500 Real-Time PCR System (Applied Biosystems, USA), according to the manufacturer's instructions.
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