The E3 ligase TRIM56 is a host restriction factor of Zika virus and depends on its RNA-binding activity but not miRNA regulation, for antiviral function

Yang, Darong; Li, Nan L.; Wei, Dahai; Liu, Baoming; Guo, Fang; Elbahesh, Husni; Zhang, Yunzhi; Zhou, Zhi; Chen, Guo-Yun; Li, Kui

Human embryonic kidney (HEK) 293, HeLa, SV40 T antigen-transformed human fetal glial cell line SVGA (a gift from Santosh Kumar, University of Tennessee Health Science Center (UTHSC)), human neuroblastoma SK-N-SH (kindly provided by Francesca-Fang Liao, UTHSC), human hepatoma cell lines Huh7 and HLCZ01 (kindly provided by Haizhen Zhu, Hunan University, China) [32] , SV40 T antigen-immortalized human hepatocyte PH5CH8, African green monkey kidney cell lines Vero and BSC-1, murine embryonic fibroblasts (MEFs), mouse fibroblast cell line L929, mouse hepatoma Hepa1-6 cells, and mosquito C6/36 cells were maintained in Dulbecco's modified Eagle medium (DMEM) supplemented with 10% fetal bovine serum (FBS), 100 U/ml of penicillin, and 100 μg/ml of streptomycin. HEK293T and its clonal derivatives deficient in Dicer expression, No-Dice (2) (3) (4) (5) (6) (7) (8) (9) (10) (11) (12) (13) (14) (15) (16) (17) (18) (19) (20) and No-Dice (4-25) [33] , were kindly provided by Bryan Cullen (Duke University Medical Center).