Author: Mathew, Suneeth F.; Crowe-McAuliffe, Caillan; Graves, Ryan; Cardno, Tony S.; McKinney, Cushla; Poole, Elizabeth S.; Tate, Warren P.
Title: The Highly Conserved Codon following the Slippery Sequence Supports -1 Frameshift Efficiency at the HIV-1 Frameshift Site Document date: 2015_3_25
ID: 10p3mth2_29
Snippet: As the shRNA technology aimed at reducing eRF1 concentrations caused unexplained effects for the frameshifting assay, eRF1 was instead over-expressed in cells with an alternative technology. First, to establish that the endogenous eRF1 concentration was not saturating and that over-expressed eRF1 was active in the cultured cells, the stop codon readthrough reporter system was utilised as before. Subsequently, frameshifting at the antizyme +1 PRF .....
Document: As the shRNA technology aimed at reducing eRF1 concentrations caused unexplained effects for the frameshifting assay, eRF1 was instead over-expressed in cells with an alternative technology. First, to establish that the endogenous eRF1 concentration was not saturating and that over-expressed eRF1 was active in the cultured cells, the stop codon readthrough reporter system was utilised as before. Subsequently, frameshifting at the antizyme +1 PRF element and the HIV −1 PRF element were tested.
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