Author: Kremer, Melanie; Suezer, Yasemin; Volz, Asisa; Frenz, Theresa; Majzoub, Monir; Hanschmann, Kay-Martin; Lehmann, Michael H.; Kalinke, Ulrich; Sutter, Gerd
Title: Critical Role of Perforin-dependent CD8+ T Cell Immunity for Rapid Protective Vaccination in a Murine Model for Human Smallpox Document date: 2012_3_1
ID: 0mmtcbof_42
Snippet: Intradermal (i.d.) vaccination was performed by tail scarification as described elsewhere [41] . Briefly, 10 ml of virus suspension containing 10 6 PFU VACV Elstree was deposited on the mouse skin at the tail base. The skin was then scratched through the droplet with the tip of a 26-gauge needle (Braun, Melsungen, Germany) to allow virus uptake. For intramuscular (i.m.) vaccination, 50 ml of virus suspension containing 10 8 PFU of MVA or PBS as a.....
Document: Intradermal (i.d.) vaccination was performed by tail scarification as described elsewhere [41] . Briefly, 10 ml of virus suspension containing 10 6 PFU VACV Elstree was deposited on the mouse skin at the tail base. The skin was then scratched through the droplet with the tip of a 26-gauge needle (Braun, Melsungen, Germany) to allow virus uptake. For intramuscular (i.m.) vaccination, 50 ml of virus suspension containing 10 8 PFU of MVA or PBS as a mock control were injected into the right hind leg. Intranasal (i.n.) immunization was performed as described elsewhere [24, 43] . Briefly, mice were anesthetized by intraperitoneal (i.p.) injection with 1 mg ketamine and 0.04 mg xylazine per 10 g body weight and instilled i.n. with 30 ml of virus suspension containing 10 8 PFU of MVA. In all experiments inoculations of corresponding amounts of PBS were used as controls (mock vaccine).
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