Selected article for: "anti mouse and optical density"
Author: Kremer, Melanie; Suezer, Yasemin; Volz, Asisa; Frenz, Theresa; Majzoub, Monir; Hanschmann, Kay-Martin; Lehmann, Michael H.; Kalinke, Ulrich; Sutter, Gerd
Title: Critical Role of Perforin-dependent CD8+ T Cell Immunity for Rapid Protective Vaccination in a Murine Model for Human Smallpox
  • Document date: 2012_3_1
    • ID: 0mmtcbof_57
    Snippet: ELISA plates (MaxiSorp 96-well flat-bottom, Nunc, Wiesbaden, Germany) were coated with sucrose gradient-purified MVA (at a protein concentration of 1 mg/ml) for 3 h at 37uC and overnight at 4uC. The plates were blocked with PBS containing 0.05% Tween 20 and 10% fetal calf serum for 60 min at 37uC. BAL fluids were incubated for 60 min at 37uC, washed five times with PBS, and then incubated for 30 min with a goat anti-mouse IgG conjugated to horser.....
    Document: ELISA plates (MaxiSorp 96-well flat-bottom, Nunc, Wiesbaden, Germany) were coated with sucrose gradient-purified MVA (at a protein concentration of 1 mg/ml) for 3 h at 37uC and overnight at 4uC. The plates were blocked with PBS containing 0.05% Tween 20 and 10% fetal calf serum for 60 min at 37uC. BAL fluids were incubated for 60 min at 37uC, washed five times with PBS, and then incubated for 30 min with a goat anti-mouse IgG conjugated to horseradish peroxidase (HRP) (Kirkegaard & Perry Laboratories, Gaithersburg USA) (diluted 1:2000 in PBS). After five washes, the plates were incubated with OPD substrate (Sigma, Taufkirchen, Germany) at room temperature for 5-10 min. The optical density was measured immediately after addition of stop solution (0.5 M sulfuric acid) at a wavelength of 490 nm [94] .

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