Selected article for: "luciferase assay buffer and lysis buffer"

Author: Shields, Lauren E.; Jennings, Jordan; Liu, Qinfang; Lee, Jinhwa; Ma, Wenjun; Blecha, Frank; Miller, Laura C.; Sang, Yongming
Title: Cross-Species Genome-Wide Analysis Reveals Molecular and Functional Diversity of the Unconventional Interferon-? Subtype
  • Document date: 2019_6_25
  • ID: 14gcu1se_22
    Snippet: IFN bioassays were conducted in MARC-145 cells stably transformed with IRF3-, IRF7-, or Mx1-promoter driven luciferase reporter systems (9, 13, 22) . In brief, for bioassays, MARC-145 (IRF3, IRF7, or Mx1) cells were treated with IFN peptides at indicated concentrations for 24 h, lysed with Glo lysis buffer and quantified by Steady-Glo R Luciferase Assay System (Promega) (13, 23) ......
    Document: IFN bioassays were conducted in MARC-145 cells stably transformed with IRF3-, IRF7-, or Mx1-promoter driven luciferase reporter systems (9, 13, 22) . In brief, for bioassays, MARC-145 (IRF3, IRF7, or Mx1) cells were treated with IFN peptides at indicated concentrations for 24 h, lysed with Glo lysis buffer and quantified by Steady-Glo R Luciferase Assay System (Promega) (13, 23) .

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