Author: Guo, Huichen; Huang, Mei; Yuan, Quan; Wei, Yanquan; Gao, Yuan; Mao, Lejiao; Gu, Lingjun; Tan, Yong Wah; Zhong, Yanxin; Liu, Dingxiang; Sun, Shiqi
Title: The Important Role of Lipid Raft-Mediated Attachment in the Infection of Cultured Cells by Coronavirus Infectious Bronchitis Virus Beaudette Strain Document date: 2017_1_12
ID: 0238bsxb_13
Snippet: Vero cell monolayers were washed with serum-free medium. The cells were infected with IBV of 1 MOI for 1 h at 4˚C before or after MβCD or Mevastatin treatment at 4˚C for 1 h. Drug concentration was determined from the dose-response curve. The cells were washed twice with serum-free medium and then grown in serum-free medium for different periods. Cell culture supernatants and whole cells were harvested to detect viral genes or proteins by RT-P.....
Document: Vero cell monolayers were washed with serum-free medium. The cells were infected with IBV of 1 MOI for 1 h at 4˚C before or after MβCD or Mevastatin treatment at 4˚C for 1 h. Drug concentration was determined from the dose-response curve. The cells were washed twice with serum-free medium and then grown in serum-free medium for different periods. Cell culture supernatants and whole cells were harvested to detect viral genes or proteins by RT-PCR or Western blot analysis, respectively.
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