Author: Guo, Huichen; Huang, Mei; Yuan, Quan; Wei, Yanquan; Gao, Yuan; Mao, Lejiao; Gu, Lingjun; Tan, Yong Wah; Zhong, Yanxin; Liu, Dingxiang; Sun, Shiqi
Title: The Important Role of Lipid Raft-Mediated Attachment in the Infection of Cultured Cells by Coronavirus Infectious Bronchitis Virus Beaudette Strain Document date: 2017_1_12
ID: 0238bsxb_17
Snippet: Vero cell monolayers that adhered to coverslips were transfected with plasmids encoding IBV E protein and were then incubated at 37ËšC for 8, 16, and 24 h. The cells were fixed with 4% paraformaldehyde (PFA) and treated with 0.1% Triton X-100. The cells were incubated with anti-IBV E protein or GM1 antibodies at 37ËšC for 1 h. Immunofluorescence confocal microscopy images were captured with LSM510 META microscope (Zeiss, Jena, Germany)......
Document: Vero cell monolayers that adhered to coverslips were transfected with plasmids encoding IBV E protein and were then incubated at 37ËšC for 8, 16, and 24 h. The cells were fixed with 4% paraformaldehyde (PFA) and treated with 0.1% Triton X-100. The cells were incubated with anti-IBV E protein or GM1 antibodies at 37ËšC for 1 h. Immunofluorescence confocal microscopy images were captured with LSM510 META microscope (Zeiss, Jena, Germany).
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