BAR scaffolds drive membrane fission by crowding disordered domains

Wilton T. Snead; Wade F. Zeno; Grace Kago; Ryan W. Perkins; J Blair Richter; Chi Zhao; Eileen M. Lafer; Jeanne C. Stachowiak

Similarly, tethered vesicle fission experiments revealed that FCHo1-FL drove potent membrane fission over the concentration range of 10-250 nM (Fig. 5I,K) , a comparable range to Amph-FL (50-150 nM, Fig. 2D ,F), with a mean diameter of 19±1 nm s.e.m. at 250 nM of FCHo1-FL. However, the F-BAR domain alone did not drive fission in these experiments, even at substantially higher concentrations up to 2 µM (Fig. 5J,K) . Interestingly, in both tethered vesicle and TEM experiments, the fission products produced by FCHo1-FL had a peak diameter of slightly less than 20 nm, with some vesicles as small as 10 nm. These small diameters suggest that FCHo1-FL may stabilize All rights reserved. No reuse allowed without permission.