Author: Law, Helen KW; Cheung, Chung Yan; Sia, Sin Fun; Chan, Yuk On; Peiris, JS Malik; Lau, Yu Lung
Title: Toll-like receptors, chemokine receptors and death receptor ligands responses in SARS coronavirus infected human monocyte derived dendritic cells Document date: 2009_6_8
ID: 19noki6p_34
Snippet: CD14+ monocytes were cultured in the presence of IL-4 (10 ng/ml; R&D, Minneapolis, USA) and GM-CSF (50 ng/ ml; R&D, Minneapolis, USA) for 7 days at 37°C in a humidified atmosphere containing 5% CO 2 as in our previous study [8] . The cultures were fed with fresh medium and cytokines on Day 3 and cell differentiation was monitored by light microscopy. On Day 5, DCs were harvested, centrifuged, washed and adjusted to 1 × 10 6 cells/ mL before vir.....
Document: CD14+ monocytes were cultured in the presence of IL-4 (10 ng/ml; R&D, Minneapolis, USA) and GM-CSF (50 ng/ ml; R&D, Minneapolis, USA) for 7 days at 37°C in a humidified atmosphere containing 5% CO 2 as in our previous study [8] . The cultures were fed with fresh medium and cytokines on Day 3 and cell differentiation was monitored by light microscopy. On Day 5, DCs were harvested, centrifuged, washed and adjusted to 1 × 10 6 cells/ mL before virus infection.
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