Transcriptome Analysis of Human Peripheral Blood Mononuclear Cells Exposed to Lassa Virus and to the Attenuated Mopeia/Lassa Reassortant 29 (ML29), a Vaccine Candidate

Zapata, Juan Carlos; Carrion, Ricardo; Patterson, Jean L.; Crasta, Oswald; Zhang, Yan; Mani, Sachin; Jett, Marti; Poonia, Bhawna; Djavani, Mahmoud; White, David M.; Lukashevich, Igor S.; Salvato, Maria S.

Table S1 A total of 122 genes were identified as being differentially expressed at tested time-points. 136 appear in the table due to the use of more than one probe to detect the same gene. Column A shows the Affymetrix gene name. Column B corresponds to the probe identification reference. Columns C to E are the comparison between LASV exposure and un-exposed cells at different time points. Columns F to H are the comparison between ML29 and un-exposed cells at different time points. Column I has the Unigene ID numbers, and column J has the GenBank accession numbers. The numbers indicate fold-changes in gene expression when compared with the control. Red color represents the more significant upregulated genes. Green color represents the more significant down-regulated genes. (TIF ) Table S2 SAM ANALYSIS of the RNA profiles of PBMC exposed to LASV and ML29 in vitro compared to RNA profiles of PBMC from WE/ARM infected monkeys. ,140 genes with common patterns and ,7 with different patterns, all at 24 hpe. The navy blue highlighting indicates the discrepancies in transcriptome between the LASV/ML29 pair and the LCMV-WE/ARM pair. Yellow highlighting indicates interferon pathway genes and coagulation-related genes. (TIF) Author Contributions