Transfer of Anti-Rotavirus Antibodies during Pregnancy and in Milk Following Maternal Vaccination with a Herpes Simplex Virus Type-1 Amplicon Vector

Meier, Anita F.; Suter, Mark; Schraner, Elisabeth M.; Humbel, Bruno M.; Tobler, Kurt; Ackermann, Mathias; Laimbacher, Andrea S.

Enzyme-linked immunosorbent assay measurements were analyzed as follows: The mean of each sample triplicate was calculated and the mean value of the negative control from the same plate was subtracted. To make values between the plates comparable, always the same positive control was applied on each plate. The mean of each sample triplicate was then set in relation to the mean of the positive control (100%) from the individual plate. A two-way ANOVA with weighted means was performed to calculate the p-values. For samples of Figure 3D , Tukey ad hoc testing was done to determine the p-values between all groups. Pre-processing of data (normalization, calculation of means and standard deviations) was performed with Microsoft Excel 2011 (Version 14.1.0). Two-way ANOVA with weighted means and Tukey ad hoc testing were performed using the open source program RStudio (Version 0.99.903-2009-2016 RStudio, Inc., Boston, MA, USA). Graphs were generated with RStudio.