Author: Qian, Shaoju; Gao, Zitong; Cao, Rui; Yang, Kang; Cui, Yijie; Li, Shaowen; Meng, Xianrong; He, Qigai; Li, Zili
Title: Transmissible Gastroenteritis Virus Infection Up-Regulates FcRn Expression via Nucleocapsid Protein and Secretion of TGF-ß in Porcine Intestinal Epithelial Cells Document date: 2020_1_21
ID: 06qddkw0_20
Snippet: In order to determine whether TGEV induces FcRn expression in IPEC-J2 cells, live or UV-inactivated TGEV at a multiplicity of infection (MOI) of 1 was used to inoculate IPEC-J2 cells, which were then harvested for FcRn analysis at 12, 24, 36, and 48 hpi by RT-qPCR and Western blot (Figures 1B,C) . TGEV induced FcRn mRNA expression about two fold higher at 24, 36, and 48 hpi, and the elevation in protein levels correlated with the increased mRNA. .....
Document: In order to determine whether TGEV induces FcRn expression in IPEC-J2 cells, live or UV-inactivated TGEV at a multiplicity of infection (MOI) of 1 was used to inoculate IPEC-J2 cells, which were then harvested for FcRn analysis at 12, 24, 36, and 48 hpi by RT-qPCR and Western blot (Figures 1B,C) . TGEV induced FcRn mRNA expression about two fold higher at 24, 36, and 48 hpi, and the elevation in protein levels correlated with the increased mRNA. However, UV-inactivated viral treatment resulted in only a 1.3-fold up-regulation of FcRn expression compared with that in simulated infected cells, indicating that the replicating virus is more effective than the UV-inactivated virus.
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