Author: Wilton T. Snead; Wade F. Zeno; Grace Kago; Ryan W. Perkins; J Blair Richter; Chi Zhao; Eileen M. Lafer; Jeanne C. Stachowiak
Title: BAR scaffolds drive membrane fission by crowding disordered domains Document date: 2018_3_4
ID: drqseaaa_71
Snippet: Fission experiments were performed by mixing vesicles at a concentration of 10 µM with unlabeled protein at specified concentrations in the above MOPS buffer with 5 mM TCEP. The mixture was incubated at 37 °C for either 30 min (Amph-FL, N-BAR, Amph CTD ∆SH3, and N-BAR-epsin CTD) or 60 min (I-BAR-AP180 CTD, FCHo1-FL, and F-BAR)......
Document: Fission experiments were performed by mixing vesicles at a concentration of 10 µM with unlabeled protein at specified concentrations in the above MOPS buffer with 5 mM TCEP. The mixture was incubated at 37 °C for either 30 min (Amph-FL, N-BAR, Amph CTD ∆SH3, and N-BAR-epsin CTD) or 60 min (I-BAR-AP180 CTD, FCHo1-FL, and F-BAR).
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