Author: Yuan, Yuan; Cao, Duanfang; Zhang, Yanfang; Ma, Jun; Qi, Jianxun; Wang, Qihui; Lu, Guangwen; Wu, Ying; Yan, Jinghua; Shi, Yi; Zhang, Xinzheng; Gao, George F.
Title: Cryo-EM structures of MERS-CoV and SARS-CoV spike glycoproteins reveal the dynamic receptor binding domains Document date: 2017_4_10
ID: 094lgjnn_29
Snippet: During 2D classification of MERS-CoV S protein data, some of the class-averaged images had a hole in the middle of the protein. We selected the particles (B60,000) within these classes for the reconstruction of S1 trimers. These class-averaged images were used to build an initial model of S1 trimer for 3D classification by e2initialmodel.py program 45 . After 3D classification, B15,500 particles were kept for the high-resolution refinement imposi.....
Document: During 2D classification of MERS-CoV S protein data, some of the class-averaged images had a hole in the middle of the protein. We selected the particles (B60,000) within these classes for the reconstruction of S1 trimers. These class-averaged images were used to build an initial model of S1 trimer for 3D classification by e2initialmodel.py program 45 . After 3D classification, B15,500 particles were kept for the high-resolution refinement imposing the three-fold symmetry which resulted in a 9.5 Ã… map of S1 trimer. We were not able to identified separate S2 proteins probably because S2 trimer was lacking of a stable conformation.
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