Author: Zheng, Jie; Tan, Boon Huan; Sugrue, Richard; Tang, Kai
Title: Current Approaches on Viral Infection: Proteomics and Functional Validations Document date: 2012_11_16
ID: 1grbdlib_13
Snippet: To overcome such limitations, more accurate assessments are required to functionally validate the active state of viral or host proteins identified by MS. In this way, functional validations, which highlight proteins involved in direct cellular responses upon virus infections, would provide novel insights to proteomic datasets. These approaches involve drug inhibition of secretory pathway, immunofluorescence staining, dominant negative mutant of .....
Document: To overcome such limitations, more accurate assessments are required to functionally validate the active state of viral or host proteins identified by MS. In this way, functional validations, which highlight proteins involved in direct cellular responses upon virus infections, would provide novel insights to proteomic datasets. These approaches involve drug inhibition of secretory pathway, immunofluorescence staining, dominant negative mutant of protein target, microarrays, small interfering siRNAmediated knockdown, molecular cloning studies, or activity-based protein profiling (ABPP) (Figure 1) . In parallel to our previous review stressing global proteomic studies on virus and host interaction (Zheng et al., 2011) , this review mainly focuses on the diverse activity-based applications to validate distinctive proteomic datasets concerning pathogen-host interactions.
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