Current Approaches on Viral Infection: Proteomics and Functional Validations

Zheng, Jie; Tan, Boon Huan; Sugrue, Richard; Tang, Kai

(2008) utilized quantitative proteomics to characterize the differently expressed protein profiles upon the presence of differentially viral components of hepatitis delta virus (HDV). Huh7 cells were transiently transfected with plasmids coding S-HDAg, L-HDAg, gRNA, and agRNA, respectively, and changes in the Huh7 cell proteome were measured by 2D gel and MALDI TOF/TOF MS. Among those differentially expressed proteins, hnRNP D, HSP105, and triosephosphate isomerase were down-regulated and confirmed by RT-PCR. This work was the first to present the overall host alternations upon expression of HDV proteins and genes, thus providing clues to unravel detailed HDV replication mechanisms. Similar approaches have been used to quantify the proteome changes upon expression of exogenous small hepatitis B surface antigen (SHBs) in HepG2 cells , recombinant influenza viral protein fused with HIV-1 p17 protein in CD8+ T cells (de Goede et al., 2009 ).