Author: Selinger, Christian; Tisoncik-Go, Jennifer; Menachery, Vineet D; Agnihothram, Sudhakar; Law, G Lynn; Chang, Jean; Kelly, Sara M; Sova, Pavel; Baric, Ralph S; Katze, Michael G
Title: Cytokine systems approach demonstrates differences in innate and pro-inflammatory host responses between genetically distinct MERS-CoV isolates Document date: 2014_12_22
ID: 0y3m47lh_6
Snippet: Using a human airway cell culture model, we sought to understand which specific signaling events would be determinant components of the host response to MERS-CoV infection. We took a genomics-based approach and assessed the whole transcriptome by microarray analysis to 1) topologically characterize the kinetic and magnitudinal changes in the host response elicited by MERS-CoV Eng 1 and MERS-CoV SA 1 and 2) identify contrasting genes between the t.....
Document: Using a human airway cell culture model, we sought to understand which specific signaling events would be determinant components of the host response to MERS-CoV infection. We took a genomics-based approach and assessed the whole transcriptome by microarray analysis to 1) topologically characterize the kinetic and magnitudinal changes in the host response elicited by MERS-CoV Eng 1 and MERS-CoV SA 1 and 2) identify contrasting genes between the two viruses related to innate and proinflammatory signal stimulation. Utilizing cytokine treatment transcriptomic data sets derived from the same model system, we pursued cytokine signaling events in MERS-CoV-infected Calu-3 cells driving statistically significant contrasting gene expression observed between MERS-CoV Eng 1 and MERS-CoV SA 1. On the basis of the viruscontrasting genes, we predicted STAT3 as a regulator of MERS-CoV-induced host responses, with strain-specific differences in STAT3-mediated gene expression.
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