Selected article for: "positive control and template control"

Author: Huang, Huanhuan; Chen, Suqing; Zhang, Xiaoyan; Hong, Linliang; Zeng, Yongbin; Wu, Bin
Title: Detection and clinical characteristics analysis of respiratory viruses in hospitalized children with acute respiratory tract infections by a GeXP-based multiplex-PCR assay
  • Document date: 2019_11_27
  • ID: 1pxki8y8_17
    Snippet: RNA-free water and the plasmid carrying the corresponding viral nucleic acid were used as a no-template control (NTC) and positive control (PC), respectively. As indicated in Figure 1 , no corresponding peak except the internal reference was observed in the NTC. Whereas 11 virus peaks appeared in the PC, and the peaks were well separated. No crossover reaction was found in PC. These indicated that the results of GeXP-based assay were accurate and.....
    Document: RNA-free water and the plasmid carrying the corresponding viral nucleic acid were used as a no-template control (NTC) and positive control (PC), respectively. As indicated in Figure 1 , no corresponding peak except the internal reference was observed in the NTC. Whereas 11 virus peaks appeared in the PC, and the peaks were well separated. No crossover reaction was found in PC. These indicated that the results of GeXP-based assay were accurate and reliable.

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