Author: Patton, John B.; Bennuru, Sasisekhar; Eberhard, Mark L.; Hess, Jessica A.; Torigian, April; Lustigman, Sara; Nutman, Thomas B.; Abraham, David
Title: Development of Onchocerca volvulus in humanized NSG mice and detection of parasite biomarkers in urine and serum Document date: 2018_12_12
ID: 0yh5k6jk_21
Snippet: Two approaches were used to create humanized mice containing multiple human-cell types [25, 36] . Human umbilical cord blood was obtained through collaboration with Thomas Jefferson University Hospital Department of Obstetrics and Gynecology from full term natural deliveries. CD34 + stem cells were isolated from cord blood using magnetic assisted cell sorting (MACS) and cryopreserved until use. SGM, NRG and NSG mice were humanized with CD34 + umb.....
Document: Two approaches were used to create humanized mice containing multiple human-cell types [25, 36] . Human umbilical cord blood was obtained through collaboration with Thomas Jefferson University Hospital Department of Obstetrics and Gynecology from full term natural deliveries. CD34 + stem cells were isolated from cord blood using magnetic assisted cell sorting (MACS) and cryopreserved until use. SGM, NRG and NSG mice were humanized with CD34 + umbilical cord derived stem cells by intrahepatic injection of 5x10 5 CD34 + stem cells into 48-hour old pups that were irradiated with 1.5 gray. Six weeks following injection of the stem cells, peripheral blood from the mice was screened for the presence of human cells and mice with counts greater than 600 human CD45 + hematopoietic cells per μl of whole blood were used for experimentation, following previously published protocols [31] .
Search related documents:
Co phrase search for related documents- blood μl and human cell: 1, 2, 3, 4, 5, 6
- blood μl and mice peripheral blood: 1, 2
- blood μl and peripheral blood: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73
- blood μl and stem cell: 1
- blood μl and umbilical cord blood: 1
- cell sort and human cell: 1
- cell sort and peripheral blood: 1
- cord blood and hematopoietic cell: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18
- cord blood and human cell: 1, 2, 3, 4, 5, 6
- cord blood and human umbilical cord blood: 1, 2, 3, 4, 5, 6, 7
- cord blood and mice peripheral blood: 1, 2, 3
- cord blood and peripheral blood: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36
- cord blood and stem cell: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34
- cord blood and umbilical cord blood: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72
Co phrase search for related documents, hyperlinks ordered by date