Author: Hayward, Joshua A; Tachedjian, Mary; Cui, Jie; Cheng, Adam Z; Johnson, Adam; Baker, Michelle L; Harris, Reuben S; Wang, Lin-Fa; Tachedjian, Gilda
Title: Differential Evolution of Antiretroviral Restriction Factors in Pteropid Bats as Revealed by APOBEC3 Gene Complexity Document date: 2018_3_29
ID: 1i6c0l3e_37
Snippet: Human embryonic kidney cells (293 T cells) and human epithelial cervical adenocarcinoma cells modified to express constitutively high levels of HIV-1 receptors and coreceptors (TZM-bl cells), were utilized in the inhibition of infectivity assay. The TZM-bl cells were obtained through the NIH AIDS Research and Reference Reagent Program (Wei et al. 2002) . All cell cultures were maintained at 37 C, 5% CO 2 in Dulbecco's modified Eagle's medium (The.....
Document: Human embryonic kidney cells (293 T cells) and human epithelial cervical adenocarcinoma cells modified to express constitutively high levels of HIV-1 receptors and coreceptors (TZM-bl cells), were utilized in the inhibition of infectivity assay. The TZM-bl cells were obtained through the NIH AIDS Research and Reference Reagent Program (Wei et al. 2002) . All cell cultures were maintained at 37 C, 5% CO 2 in Dulbecco's modified Eagle's medium (Thermo) supplemented with heat-inactivated fetal bovine serum (100 ml/l; Invitrogen), glutamine (292 lg/ml; Invitrogen), and the antibiotics penicillin (100 units/ml; Invitrogen) and streptomycin (100 units/ml; Invitrogen).
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