Author: Nakamura, Shota; Yang, Cheng-Song; Sakon, Naomi; Ueda, Mayo; Tougan, Takahiro; Yamashita, Akifumi; Goto, Naohisa; Takahashi, Kazuo; Yasunaga, Teruo; Ikuta, Kazuyoshi; Mizutani, Tetsuya; Okamoto, Yoshiko; Tagami, Michihira; Morita, Ryoji; Maeda, Norihiro; Kawai, Jun; Hayashizaki, Yoshihide; Nagai, Yoshiyuki; Horii, Toshihiro; Iida, Tetsuya; Nakaya, Takaaki
Title: Direct Metagenomic Detection of Viral Pathogens in Nasal and Fecal Specimens Using an Unbiased High-Throughput Sequencing Approach Document date: 2009_1_19
ID: 1j8z8lak_29
Snippet: Quantitative RT-PCR of norovirus RNA extraction was performed using a Magtration-Maga-ZorbRNA Common kit (Precision System Science) and the viral copy number of norovirus was estimated with One-step real time RT-PCR [26] using a One-Step Realtime PCR reagent kit (Toyobo). A plasmid containing the target sequence was used as a control......
Document: Quantitative RT-PCR of norovirus RNA extraction was performed using a Magtration-Maga-ZorbRNA Common kit (Precision System Science) and the viral copy number of norovirus was estimated with One-step real time RT-PCR [26] using a One-Step Realtime PCR reagent kit (Toyobo). A plasmid containing the target sequence was used as a control.
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