Author: Thomas, Joseph T.; Chen, Qi; Gauger, Phillip C.; Giménez-Lirola, Luis G.; Sinha, Avanti; Harmon, Karen M.; Madson, Darin M.; Burrough, Eric R.; Magstadt, Drew R.; Salzbrenner, Holly M.; Welch, Michael W.; Yoon, Kyoung-Jin; Zimmerman, Jeffrey J.; Zhang, Jianqiang
Title: Effect of Porcine Epidemic Diarrhea Virus Infectious Doses on Infection Outcomes in Naïve Conventional Neonatal and Weaned Pigs Document date: 2015_10_6
ID: 1kjk404o_52
Snippet: In summary, using a U.S. PEDV prototype isolate diluted in culture medium, we determined the correlations of PEDV infectious titers to PCR Ct values and genomic copies per ml. We further demonstrated that PEDV infectious dose is age-dependent with a significantly lower MID for neonatal pigs than for weaned pigs. This information should be taken into consideration when interpreting clinical relevance of PEDV PCR results and when designing a PEDV b.....
Document: In summary, using a U.S. PEDV prototype isolate diluted in culture medium, we determined the correlations of PEDV infectious titers to PCR Ct values and genomic copies per ml. We further demonstrated that PEDV infectious dose is age-dependent with a significantly lower MID for neonatal pigs than for weaned pigs. This information should be taken into consideration when interpreting clinical relevance of PEDV PCR results and when designing a PEDV bioassay model. The observation of such a low MID in neonates also emphasizes the importance of strict biosecurity and thorough cleaning/disinfection on sow farms. In this study, the effect of PEDV infectious doses on infection outcomes was evaluated in naïve conventional neonatal and weaned pigs. It must be noted that infectious dose effects in previously exposed or vaccinated pigs may be different from naïve pigs. In addition, a U.S. PEDV prototype cell culture isolate was used in this study and it remains to be determined whether or not U.S. PEDV S-INDEL-variant isolate has similar minimum infectious dose and pathogenicity.
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