Author: Mordecai, Gideon J; Miller, Kristina M; Di Cicco, Emiliano; Schulze, Angela D; Kaukinen, Karia H; Ming, Tobi J; Li, Shaorong; Tabata, Amy; Teffer, Amy; Patterson, David A; Ferguson, Hugh W; Suttle, Curtis A
Title: Endangered wild salmon infected by newly discovered viruses Document date: 2019_9_3
ID: 010xj69x_34
Snippet: The 96.96 gene expression dynamic array (Fluidigm Corporation, CA, US) was run according to the procedure outlined previously (Miller et al., 2016) . Specifically, a 5 ml template mixture was prepared for each sample containing 1 Â TaqMan Universal Master Mix (No UNG), 1 Â GE Sample Loading Reagent (Fluidigm PN 85000746) and each of diluted STA'd sample mixtures. Five ml of Assay mix was prepared with 1 Â each of the appropriate TaqMan qPCR as.....
Document: The 96.96 gene expression dynamic array (Fluidigm Corporation, CA, US) was run according to the procedure outlined previously (Miller et al., 2016) . Specifically, a 5 ml template mixture was prepared for each sample containing 1 Â TaqMan Universal Master Mix (No UNG), 1 Â GE Sample Loading Reagent (Fluidigm PN 85000746) and each of diluted STA'd sample mixtures. Five ml of Assay mix was prepared with 1 Â each of the appropriate TaqMan qPCR assays (agent probe in FAM-MGB and artificial positive construct (APC) probe in NED-MGB, 10 mM of primers and 3 mM of probes) and 1 Â Assay Loading Reagent (Fluidigm PN 85000736).
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