Author: Fongaro, Gislaine; Nascimento, Mariana A do; Rigotto, Caroline; Ritterbusch, Giseli; da Silva, Alessandra D’ A; Esteves, Paulo A; Barardi, Célia R M
Title: Evaluation and molecular characterization of human adenovirus in drinking water supplies: viral integrity and viability assays Document date: 2013_5_28
ID: 19aj551d_31
Snippet: The extraction of viral nucleic acid was performed using a commercial RTP DNA/RNA Virus® II Mini Kit (Invitek), according to the manufacturer's instructions. Nucleic acid was eluted in a 60 μL elution buffer and stored at −80°C until further analysis. A reverse transcriptase (RT) reaction was performed to generate cDNA from mRNA, using an RT enzyme and random primers (Sensiscript RT Kit -QIAGEN®)......
Document: The extraction of viral nucleic acid was performed using a commercial RTP DNA/RNA Virus® II Mini Kit (Invitek), according to the manufacturer's instructions. Nucleic acid was eluted in a 60 μL elution buffer and stored at −80°C until further analysis. A reverse transcriptase (RT) reaction was performed to generate cDNA from mRNA, using an RT enzyme and random primers (Sensiscript RT Kit -QIAGEN®).
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