Author: Fongaro, Gislaine; Nascimento, Mariana A do; Rigotto, Caroline; Ritterbusch, Giseli; da Silva, Alessandra D’ A; Esteves, Paulo A; Barardi, Célia R M
Title: Evaluation and molecular characterization of human adenovirus in drinking water supplies: viral integrity and viability assays Document date: 2013_5_28
ID: 19aj551d_48
Snippet: Samples containing particles of infectious HAdV were selected for sequencing and molecular characterization. The primers described by Allard et al. (1992) [46] , directed to a region of the viral genome coding for the capsid hexon protein (18858-19158 bp position in HAdV genome) common to all HAdVs, were used for PCR, generating a product of approximately 300 bp. The amplicons were purified with ExoSAP-IT (GE Healthcare UK Ltd, Buckinghamshire, U.....
Document: Samples containing particles of infectious HAdV were selected for sequencing and molecular characterization. The primers described by Allard et al. (1992) [46] , directed to a region of the viral genome coding for the capsid hexon protein (18858-19158 bp position in HAdV genome) common to all HAdVs, were used for PCR, generating a product of approximately 300 bp. The amplicons were purified with ExoSAP-IT (GE Healthcare UK Ltd, Buckinghamshire, UK). Ultra-pure water was used as the non-template control for each assay.
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