Author: Zhang, Ying; Cao, Lan; Xu, Zhi; Zhu, Pingting; Huang, Bing; Li, Kuibiao; Xu, Yang; Zhang, Zhoubin; Wu, Yong; Di, Biao
Title: Evaluation of a multiplex PCR assay for detection of respiratory viruses and Mycoplasma pneumoniae in oropharyngeal swab samples from outpatients Document date: 2019_10_19
ID: 07dpy0jn_6
Snippet: The specimen was shaken vigorously for 5 minutes in phosphatebuffered saline solution, centrifuged at 9.6 g for 20 minutes, and the supernatant was aspirated. About 50 µL of RNA was extracted from 140 µL supernatant using the QIAamp Viral RNA extraction kit (QIAGEN, Hilden, Germany), according to the manufacture's instruction and was stored at −80°C......
Document: The specimen was shaken vigorously for 5 minutes in phosphatebuffered saline solution, centrifuged at 9.6 g for 20 minutes, and the supernatant was aspirated. About 50 µL of RNA was extracted from 140 µL supernatant using the QIAamp Viral RNA extraction kit (QIAGEN, Hilden, Germany), according to the manufacture's instruction and was stored at −80°C.
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