Evaluation of the Replication, Pathogenicity, and Immunogenicity of Avian Paramyxovirus (APMV) Serotypes 2, 3, 4, 5, 7, and 9 in Rhesus Macaques

Khattar, Sunil K.; Nayak, Baibaswata; Kim, Shin-Hee; Xiao, Sa; Samal, Sweety; Paldurai, Anandan; Buchholz, Ursula J.; Collins, Peter L.; Samal, Siba K.

To evaluate replication of the APMVs in the upper respiratory tract of rhesus macaques, nasal washes were collected from days 0 to 10 and on days 12 and 21. Virus titers were determined by titration in embryonated chicken eggs, with the exception of APMV-5, which does not grow in chicken eggs. Thus, serial dilutions were prepared from the nasal washes and inoculated into the allantoic cavity of embryonated 9-day-old chicken eggs, and 4 dpi the allantoic fluid was collected and assayed for the presence of virus by HA assay. Virus titers were expressed as log 10 EID 50 per mL ( Table 2 ). Replication of APMV-5 was determined by a limiting dilution assay on Vero cells in which virus-infected wells were detected by NA activity.