Author: Drexler, Jan Felix; Corman, Victor Max; Müller, Marcel Alexander; Lukashev, Alexander N.; Gmyl, Anatoly; Coutard, Bruno; Adam, Alexander; Ritz, Daniel; Leijten, Lonneke M.; van Riel, Debby; Kallies, Rene; Klose, Stefan M.; Gloza-Rausch, Florian; Binger, Tabea; Annan, Augustina; Adu-Sarkodie, Yaw; Oppong, Samuel; Bourgarel, Mathieu; Rupp, Daniel; Hoffmann, Bernd; Schlegel, Mathias; Kümmerer, Beate M.; Krüger, Detlev H.; Schmidt-Chanasit, Jonas; Setién, Alvaro Aguilar; Cottontail, Veronika M.; Hemachudha, Thiravat; Wacharapluesadee, Supaporn; Osterrieder, Klaus; Bartenschlager, Ralf; Matthee, Sonja; Beer, Martin; Kuiken, Thijs; Reusken, Chantal; Leroy, Eric M.; Ulrich, Rainer G.; Drosten, Christian
Title: Evidence for Novel Hepaciviruses in Rodents Document date: 2013_6_20
ID: 1v353uij_24
Snippet: HCV Western blot. Western blot (WB) analysis was performed with commercially available HCV strips (recomBlot HCV IgG 2.0 and recomLine HCV IgG, Microgen, Neuried, Germany). Bat and rodent sera were diluted 1:100 for screening. Horseradish peroxidase-labelled goat anti-bat immunoglobulin (Ig) conjugate (Bethyl, Montgomery, AL, USA) or goat-anti mouse Ig (Dianova, Hamburg, Germany) were used as secondary antibodies (dilution, 1:500). For rodent WB,.....
Document: HCV Western blot. Western blot (WB) analysis was performed with commercially available HCV strips (recomBlot HCV IgG 2.0 and recomLine HCV IgG, Microgen, Neuried, Germany). Bat and rodent sera were diluted 1:100 for screening. Horseradish peroxidase-labelled goat anti-bat immunoglobulin (Ig) conjugate (Bethyl, Montgomery, AL, USA) or goat-anti mouse Ig (Dianova, Hamburg, Germany) were used as secondary antibodies (dilution, 1:500). For rodent WB, a tertiary horseradish peroxidase-labelled donkey-anti goat Ig (Santa Cruz Biotechnology, Santa Cruz, CA, USA) was used for signal amplification. Blots were evaluated following the manufacturer's instructions.
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