Author: Frenzel, André; Hust, Michael; Schirrmann, Thomas
Title: Expression of Recombinant Antibodies Document date: 2013_7_29
ID: 06o7pa3d_45
Snippet: The human embryonic kidney (HEK) 293 cell lines have been widely used for transient protein expression because they can be very efficiently transfected with plasmid DNA. Some derivatives were further transformed either with the simian virus 40 (SV40) large T antigen, termed HEK293T, or with the Epstein Barr virus (EBV) nuclear antigen 1 (EBNA1), termed HEK293E, in order to mediate semi-stable episomal propagation of vectors containing an origin o.....
Document: The human embryonic kidney (HEK) 293 cell lines have been widely used for transient protein expression because they can be very efficiently transfected with plasmid DNA. Some derivatives were further transformed either with the simian virus 40 (SV40) large T antigen, termed HEK293T, or with the Epstein Barr virus (EBV) nuclear antigen 1 (EBNA1), termed HEK293E, in order to mediate semi-stable episomal propagation of vectors containing an origin of replication (ori) of SV40 or EBV, respectively. Transient transfection of plasmid DNA in HEK293 cells can also be performed in large scale by calcium phosphate transfection (180) , cationic liposomes, and polymers like polyethyleneimine (PEI) (181, 182) .
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