Author: Ye, Fuqiang; Han, Yifang; Zhu, Juanjuan; Li, Peng; Zhang, Qi; Lin, Yanfeng; Wang, Taiwu; Lv, Heng; Wang, Changjun; Wang, Chunhui; Zhang, Jinhai
Title: First Identification of Human Adenovirus Subtype 21a in China With MinION and Illumina Sequencers Document date: 2020_4_7
ID: 18b2foud_56
Snippet: Because of the long-read output by MinION, a minimum of 500 reads covered ≥96.49% of the present genome. This implied that under resource-limited conditions we could attempt to terminate the sequencing run ahead of time. It was observed that a downsampling procedure with maximal aligned read length ≥25 kb could cover ≥69.10% of the genome. As most reads longer than 20 kb could be identified as part of the current genome, we deemed reads wit.....
Document: Because of the long-read output by MinION, a minimum of 500 reads covered ≥96.49% of the present genome. This implied that under resource-limited conditions we could attempt to terminate the sequencing run ahead of time. It was observed that a downsampling procedure with maximal aligned read length ≥25 kb could cover ≥69.10% of the genome. As most reads longer than 20 kb could be identified as part of the current genome, we deemed reads with a length of 25 kb were able to reach a genome coverage of 70.82% (25/35.3) regardless of sequencing errors.
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