Selected article for: "cc international license and II topo"
Author: Joshua A. Hayward; Mary Tachedjian; Adam Johnson; Tamsin B. Gordon; Jie Cui; Glenn A. Marsh; Michelle L. Baker; Lin-Fa Wang; Gilda Tachedjian
Title: Bats Possess Unique Variants of the Antiviral Restriction Factor Tetherin
  • Document date: 2020_4_9
    • ID: eksi9yia_65
    Snippet: To express tetherin proteins in a mammalian cell culture system the tagged tetherin inserts were sub-cloned from the pCR2.1-TOPO (P. alecto tetherin isoforms A, B, and C) and pCR-. CC-BY-NC-ND 4.0 International license author/funder. It is made available under a The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/2020.04.08.031203 doi: bioRxiv preprint Blunt-II-TOPO (M. macropus tetherin A and B) .....
    Document: To express tetherin proteins in a mammalian cell culture system the tagged tetherin inserts were sub-cloned from the pCR2.1-TOPO (P. alecto tetherin isoforms A, B, and C) and pCR-. CC-BY-NC-ND 4.0 International license author/funder. It is made available under a The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/2020.04.08.031203 doi: bioRxiv preprint Blunt-II-TOPO (M. macropus tetherin A and B) vectors into pcDNA3.1 mammalian expression vectors. The HA-tagged tetherin constructs contained terminal enzyme restriction sites. P. alecto tetherin constructs contained XhoI and XbaI sites at their 5' and 3' ends, respectively.

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