Selected article for: "blood sample and peripheral blood sample"

Author: Funaro, Ada; Gribaudo, Giorgio; Luganini, Anna; Ortolan, Erika; Lo Buono, Nicola; Vicenzi, Elisa; Cassetta, Luca; Landolfo, Santo; Buick, Richard; Falciola, Luca; Murphy, Marianne; Garotta, Gianni; Malavasi, Fabio
Title: Generation of potent neutralizing human monoclonal antibodies against cytomegalovirus infection from immune B cells
  • Document date: 2008_11_12
  • ID: 1u2fkwx3_23
    Snippet: In summary, this study provides proof of concept that good-quality, fully human mAbs with desired specificity and biological activity can be generated with the SEQUENTIAL method. The strengths of this approach are: i) it allows the selection of human monoclonal IgG to a variety of antigens, from a small sample of fresh or frozen peripheral blood, ii) it is rapid, iii) screening can be performed using a variety of assays, including functional assa.....
    Document: In summary, this study provides proof of concept that good-quality, fully human mAbs with desired specificity and biological activity can be generated with the SEQUENTIAL method. The strengths of this approach are: i) it allows the selection of human monoclonal IgG to a variety of antigens, from a small sample of fresh or frozen peripheral blood, ii) it is rapid, iii) screening can be performed using a variety of assays, including functional assays, iv) the mAbs of interest can be easily produced from the original clone as recombinant proteins suitable for clinical applications, and v) the generation of IgGsecreting polyclonal populations can be considered as a library of antibody-secreting cells that can be used to select mAbs with specificities not considered when cells were immortalized.

    Search related documents:
    Co phrase search for related documents
    • antibody secrete and peripheral blood: 1, 2
    • approach strength and small sample: 1
    • assay variety and peripheral blood: 1
    • biological activity and clinical application: 1, 2, 3
    • biological activity and concept proof: 1, 2
    • biological activity and good quality: 1
    • biological activity and peripheral blood: 1, 2, 3, 4, 5
    • biological activity and recombinant protein: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10