Author: Lu, Shuai; Chen, Yingzhu; Qin, Kun; Zhou, Jianfang; Lou, Yongliang; Tan, Wenjie
Title: Genetic and antigenic characterization of recombinant nucleocapsid proteins derived from canine coronavirus and canine respiratory coronavirus in China Document date: 2016_4_15
ID: 1ghgd7yg_22
Snippet: Viral RNA was obtained from 140 μL of clarified swab extract with a QIAamp Viral RNA mini kit (QIAGEN, Germany) following the manufacturer's instructions. One-step RT-PCR (QIAGEN) was performed to amplify the full-length N genes of CCoV and CRCoV using specific primers with enzyme cleavage sites BamH I and Xho I (Table 2). The PCR products were cloned into the pMD19-T vector and verified by sequencing. The nucleotide sequences obtained were depo.....
Document: Viral RNA was obtained from 140 μL of clarified swab extract with a QIAamp Viral RNA mini kit (QIAGEN, Germany) following the manufacturer's instructions. One-step RT-PCR (QIAGEN) was performed to amplify the full-length N genes of CCoV and CRCoV using specific primers with enzyme cleavage sites BamH I and Xho I (Table 2). The PCR products were cloned into the pMD19-T vector and verified by sequencing. The nucleotide sequences obtained were deposited to GenBank under the accession numbers KT852997 & KT852998.
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