Selected article for: "bacterial genome and reference genome GenBank file"
Author: Tcherepanov, Vasily; Ehlers, Angelika; Upton, Chris
Title: Genome Annotation Transfer Utility (GATU): rapid annotation of viral genomes using a closely related reference genome
  • Document date: 2006_6_13
    • ID: 1e2kkhht_27
    Snippet: Since many problems associated with genome annotation are magnified considerably when dealing with bacterial genomes due to their large size, we have tested GATU on bacterial genomes up to ten times the size of a typical poxvirus genome. First, Chlamydia pneumoniae strain TW183 (NC_005043) was annotated using C. pneumoniae strain AR39 (NC_002179) as the reference genome, containing 1112 annotated genes in its GenBank file. These two strains are h.....
    Document: Since many problems associated with genome annotation are magnified considerably when dealing with bacterial genomes due to their large size, we have tested GATU on bacterial genomes up to ten times the size of a typical poxvirus genome. First, Chlamydia pneumoniae strain TW183 (NC_005043) was annotated using C. pneumoniae strain AR39 (NC_002179) as the reference genome, containing 1112 annotated genes in its GenBank file. These two strains are highly similar (>90% nucleotide identity). Of the 1112 reference genes, 817 were 100% similar (global alignment with NEEDLE) to a gene found on the target genome, 145 genes were 95-99% similar, 28 genes were Results of TBLASTN search with an Unassigned-ORF; display is presented in the main GATU window Figure 5 Results of TBLASTN search with an Unassigned-ORF; display is presented in the main GATU window.

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