Selected article for: "Big dye terminator and PCR system"
Author: Vittecoq, Marion; Grandhomme, Viviane; Champagnon, Jocelyn; Guillemain, Matthieu; Crescenzo-Chaigne, Bernadette; Renaud, François; Thomas, Frédéric; Gauthier-Clerc, Michel; van der Werf, Sylvie
Title: High Influenza A Virus Infection Rates in Mallards Bred for Hunting in the Camargue, South of France
  • Document date: 2012_8_27
    • ID: 0r4z1zea_32
    Snippet: Amplification of viral RNA extracted from 4 virus isolates was carried out using a Superscript Platinum One-step RT-PCR system (Invitrogen) and primers specific of each segment. Sequencing was done using a Big Dye Terminator V1.1 kit and a sequencer ABI DNA Analyzer 3730XL (Applied Biosystems). Sequences of all primers are available upon request. Sequences of the whole viral genome were analyzed with CLC Main Workbench 5.6.1. We performed alignme.....
    Document: Amplification of viral RNA extracted from 4 virus isolates was carried out using a Superscript Platinum One-step RT-PCR system (Invitrogen) and primers specific of each segment. Sequencing was done using a Big Dye Terminator V1.1 kit and a sequencer ABI DNA Analyzer 3730XL (Applied Biosystems). Sequences of all primers are available upon request. Sequences of the whole viral genome were analyzed with CLC Main Workbench 5.6.1. We performed alignments for the 8 segments using sequences available from the Influenza Sequence Database with CLC Main Workbench 5.6.1. Phylogenetic trees were constructed using maximum parsimony (MP) methods with the dnapars program of the PHYLIP 3.68 package and the maximum likelihood (ML) with the software PhyML 2.4.4. Evolutionary model was selected using Model Generator 0.85 [58] . Nodal supports were assessed with 100 bootstrap replicates generated for each method.

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