Author: Vittecoq, Marion; Grandhomme, Viviane; Champagnon, Jocelyn; Guillemain, Matthieu; Crescenzo-Chaigne, Bernadette; Renaud, François; Thomas, Frédéric; Gauthier-Clerc, Michel; van der Werf, Sylvie
Title: High Influenza A Virus Infection Rates in Mallards Bred for Hunting in the Camargue, South of France Document date: 2012_8_27
ID: 0r4z1zea_33
Snippet: Determination of 39 and 59 End Non Coding Sequences of the 8 Segments of the H10N7 Virus Viral genomic RNA was extracted using the QIAamp Viral RNA Mini kit (Qiagen) from 140 ml of allantoic fluid according to the manufacturer's recommendations. The RNA was eluted in 60 ml of RNase-free water and the 39 and 59 NC regions were amplified as previously described [59] using an anchored (dT) 14 oligonucleotide and primers specific for the coding seque.....
Document: Determination of 39 and 59 End Non Coding Sequences of the 8 Segments of the H10N7 Virus Viral genomic RNA was extracted using the QIAamp Viral RNA Mini kit (Qiagen) from 140 ml of allantoic fluid according to the manufacturer's recommendations. The RNA was eluted in 60 ml of RNase-free water and the 39 and 59 NC regions were amplified as previously described [59] using an anchored (dT) 14 oligonucleotide and primers specific for the coding sequences of both segments for reverse transcription and amplification. After purification, the PCR products were sequenced with internal oligonucleotides using a Big Dye terminator sequencing kit and an automated sequencer (Perkin Elmer). All sequences of the primers are available from the authors upon request.
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