Two Novel Parvoviruses in Frugivorous New and Old World Bats

Canuti, Marta; Eis-Huebinger, Anna Maria; Deijs, Martin; de Vries, Michel; Drexler, Jan Felix; Oppong, Samuel K.; Müller, Marcel A.; Klose, Stefan M.; Wellinghausen, Nele; Cottontail, Veronika M.; Kalko, Elisabeth K. V.; Drosten, Christian; van der Hoek, Lia

DNA from 100 serum samples collected from Ghanaian Eidolon helvum bats was isolated by the QIAamp MinElute Virus Spin Kit (Qiagen, Hilden, Germany) according to the manufacturer's recommendations. The input volume was 200 ml, the elution volume was 120 ml. Up to 200 ml of whole EDTA blood collected from 92 Panamanian Artibeus jamaicensis bats and from 18 Panamanian Artibeus lituratus bats were mixed with 500 ml lysis buffer (10 mM Tris-HCl pH 8.0 100 mM EDTA, 2% SDS) and DNA was prepared by using the QIAamp Blood DNA Mini Kit (Qiagen, Hilden, Germany) according to the manufacturer's instructions except for an initial 15 sec incubation with proteinase K [23] . Eluate pools were then created as follows: 10 pools (10 eluates each) were prepared with 40 ml of each DNA isolate from Ghanaian Eidolon helvum serum samples and 22 pools (5 eluates each) were made with 5 ml of each DNA preparation from Panamanian Artibeus bats specimens.