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Author: Oude Munnink, Bas B; Canuti, Marta; Deijs, Martin; de Vries, Michel; Jebbink, Maarten F; Rebers, Sjoerd; Molenkamp, Richard; van Hemert, Formijn J; Chung, Kevin; Cotten, Matthew; Snijders, Fransje; Sol, Cees JA; van der Hoek, Lia
Title: Unexplained diarrhoea in HIV-1 infected individuals
  • Document date: 2014_1_13
  • ID: 0e9l19kp_43
    Snippet: Remarkably, the prevalence of Aichi virus in our study population was 20%, while previous studies only showed a prevalence of 0.5-1.6% [19, 46] . It could be that the virus is more frequently present in patients with immunodeficiency. Furthermore, the sensitivity of our assay may also play a part. In our study a very sensitive nested PCR assay was used which enables detection of the virus even if it is present at low concentrations. To exclude th.....
    Document: Remarkably, the prevalence of Aichi virus in our study population was 20%, while previous studies only showed a prevalence of 0.5-1.6% [19, 46] . It could be that the virus is more frequently present in patients with immunodeficiency. Furthermore, the sensitivity of our assay may also play a part. In our study a very sensitive nested PCR assay was used which enables detection of the virus even if it is present at low concentrations. To exclude that the high prevalence was caused by PCR contamination, all positive PCR products were sequenced. Variation between the viruses from different patients was observed, thereby excluding contamination by a single source (see nucleotide alignments in Additional file 1: Table S3 ).

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