Author: Wang, Ting; Yin, Huiquan; Li, Yan; Zhao, Lingxiao; Sun, Xiahui; Cong, Hua
Title: Vaccination with recombinant adenovirus expressing multi-stage antigens of Toxoplasma gondii by the mucosal route induces higher systemic cellular and local mucosal immune responses than with other vaccination routes Document date: 2017_4_3
ID: 1ay8y7li_37
Snippet: The antigen delivery pathway is a key parameter for the induction of protective immune responses by vaccines against intracellular pathogens [10] . As a novel inoculation, The splenocyte culture supernatants taken from mice (n = 3, each group) 2 weeks after the last immunization were stained with FITClabeled anti-mouse CD8 + monoclonal antibody and PE-labeled anti-mouse CD4 + monoclonal antibody; T lymphocyte subsets were measured using flow cyto.....
Document: The antigen delivery pathway is a key parameter for the induction of protective immune responses by vaccines against intracellular pathogens [10] . As a novel inoculation, The splenocyte culture supernatants taken from mice (n = 3, each group) 2 weeks after the last immunization were stained with FITClabeled anti-mouse CD8 + monoclonal antibody and PE-labeled anti-mouse CD4 + monoclonal antibody; T lymphocyte subsets were measured using flow cytometry. Asterisks mark the significant difference: * p < 0.05; ** p < 0.01. Each bar represents the mean OD (± SD).
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