Virome Profiling of Bats from Myanmar by Metagenomic Analysis of Tissue Samples Reveals More Novel Mammalian Viruses

He, Biao; Li, Zuosheng; Yang, Fanli; Zheng, Junfeng; Feng, Ye; Guo, Huancheng; Li, Yingying; Wang, Yiyin; Su, Nan; Zhang, Fuqiang; Fan, Quanshui; Tu, Changchun

CVs contain a circular genome of 1,700-2,000 nt length which encodes a replication-associated protein (Rep) and a capsid protein (Cap) in opposing directions [54, 55] . CVs were first discovered in American bats in 2010 and in Chinese bats in 2011 [9, 37] . In the present study, only group XO contained CV contigs (Table 3) . Further PCR confirmed this result and found that 7% (6/92) of guts of R. ferrumequinum in group XO were circovirus positive. Two complete genomes (BtCV XOR1, BtCV XOR7; accession numbers JX863737 and KC339249 respectively) were amplified by inverse PCR. Sequencing revealed that BtCV XOR1 and XOR7 DNAs were respectively 1,862 nt and 1798 nt in length and contained two ORFs in opposite strands ( Figure 6A , Table 4 ). As in other CVs, the two bat CVs had a stem-loop structure between the two ORFs with nonamer motif TAGTATTAC and TAGTATTAC, identical to that of canine CV strain NY214 (JQ821392), beak and feather disease virus, and finch CV ( Figure 6B ) (23, 32) . Moreover, three tandem copies of CGGCACA of BtCV XOR1, thought to bind the replicase when the viral DNA starts to replicate by the rolling-circle method, were present at nt 1862-26 with the highest identity to porcine CV-1 and -2 (PCV-1 and PCV-2). The tandem copies in BtCV XOR7, however, were shorter, consisting of the hexamer CGGCAG (Table 4) .