Author: Kolodziejek, Jolanta; Seidel, Bernhard; Jungbauer, Christof; Dimmel, Katharina; Kolodziejek, Michael; Rudolf, Ivo; Hubálek, Zdenek; Allerberger, Franz; Nowotny, Norbert
Title: West Nile Virus Positive Blood Donation and Subsequent Entomological Investigation, Austria, 2014 Document date: 2015_5_11
ID: 0vm2hhdr_14
Snippet: For nucleic acid extraction 140 μl of each sample was used. All extractions were performed with the QIAamp Viral RNA Mini Kit (Qiagen, USA), following the manufacturer's instructions. For detection of both WNV lineages 1 and 2 in the samples, a RT-qPCR targeting the highly conserved 5´non-coding region (NCR) was performed as described recently [4] . For confirmation and lineage determination a second RT-qPCR specific for WNV lineage 2 within th.....
Document: For nucleic acid extraction 140 μl of each sample was used. All extractions were performed with the QIAamp Viral RNA Mini Kit (Qiagen, USA), following the manufacturer's instructions. For detection of both WNV lineages 1 and 2 in the samples, a RT-qPCR targeting the highly conserved 5´non-coding region (NCR) was performed as described recently [4] . For confirmation and lineage determination a second RT-qPCR specific for WNV lineage 2 within the NS3 protein coding region was conducted as previously described [5] .
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