Author: Paesen, Guido C.; Collet, Axelle; Sallamand, Corinne; Debart, Françoise; Vasseur, Jean-Jacques; Canard, Bruno; Decroly, Etienne; Grimes, Jonathan M.
Title: X-ray structure and activities of an essential Mononegavirales L-protein domain Document date: 2015_11_9
ID: 1taxqkk2_22
Snippet: Selenomethionine incorporation. Twenty hours following infection of an Sf21 suspension culture (27.8°C, with an agitation speed of 130 r.p.m./0.25g), cells were collected (70g, 10 min, 22°C) and resuspended in cysteine-and methionine-free SF900II medium (Gibco) supplemented with dialysed fetal bovine serum (Gibco; 7% v/v) and 150 mg l À 1 L-cysteine (Sigma). Following an additional 4 h at 27.8°C in the shaking incubator, 250 mg l À 1 selenom.....
Document: Selenomethionine incorporation. Twenty hours following infection of an Sf21 suspension culture (27.8°C, with an agitation speed of 130 r.p.m./0.25g), cells were collected (70g, 10 min, 22°C) and resuspended in cysteine-and methionine-free SF900II medium (Gibco) supplemented with dialysed fetal bovine serum (Gibco; 7% v/v) and 150 mg l À 1 L-cysteine (Sigma). Following an additional 4 h at 27.8°C in the shaking incubator, 250 mg l À 1 selenomethionine (Sigma) was added. Protein expression was allowed to continue for another 48 h. Proteins were purified as above.
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