Author: Bouvette, Jonathan; Korkut, Dursun Nizam; Fouillen, Aurélien; Amellah, Soumiya; Nanci, Antonio; Durocher, Yves; Omichinski, James G.; Legault, Pascale
Title: High-yield production of human Dicer by transfection of human HEK293-EBNA1 cells grown in suspension Document date: 2018_12_6
ID: 012ipcdr_30
Snippet: HEK-293-EBNA1-6E (293-6E) cells were grown in suspension in Freestyle 17 (F17) medium (Invitrogen) supplemented with 4 mM glutamine, 0.1% pluronic acid and 25 μg/mL G418 at 37°C with 5% CO 2 . Tryptone N1 was added to some of the cell cultures at a weight/volume ratio of 0.5%, but was found to have minimal effect on cultures grown 72 hpt. Cultures were maintained under 2.0 × 10 6 cells/mL in Erlenmeyer flasks shaken at 100 RPM in a humidified .....
Document: HEK-293-EBNA1-6E (293-6E) cells were grown in suspension in Freestyle 17 (F17) medium (Invitrogen) supplemented with 4 mM glutamine, 0.1% pluronic acid and 25 μg/mL G418 at 37°C with 5% CO 2 . Tryptone N1 was added to some of the cell cultures at a weight/volume ratio of 0.5%, but was found to have minimal effect on cultures grown 72 hpt. Cultures were maintained under 2.0 × 10 6 cells/mL in Erlenmeyer flasks shaken at 100 RPM in a humidified incubator at 37°C with 5% CO 2 .
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