Author: Bouvette, Jonathan; Korkut, Dursun Nizam; Fouillen, Aurélien; Amellah, Soumiya; Nanci, Antonio; Durocher, Yves; Omichinski, James G.; Legault, Pascale
Title: High-yield production of human Dicer by transfection of human HEK293-EBNA1 cells grown in suspension Document date: 2018_12_6
ID: 012ipcdr_31
Snippet: Cell cultures were diluted to 0.8 × 10 6 cells/mL 24 h before transfection. The transfection mix was prepared in 10% of the final culture volume in F17 medium with 10 μg/mL of DNA plasmid and 20 μg/mL of linear PEI 25 kDa (Polysciences Inc.). The mix was agitated by 3 vortexing cycles of 3 s, incubated 15 min at room temperature and incorporated in the culture [32] , which was incubated for 72-96 h (37°C, 5% CO 2 ) with shaking (100 RPM)......
Document: Cell cultures were diluted to 0.8 × 10 6 cells/mL 24 h before transfection. The transfection mix was prepared in 10% of the final culture volume in F17 medium with 10 μg/mL of DNA plasmid and 20 μg/mL of linear PEI 25 kDa (Polysciences Inc.). The mix was agitated by 3 vortexing cycles of 3 s, incubated 15 min at room temperature and incorporated in the culture [32] , which was incubated for 72-96 h (37°C, 5% CO 2 ) with shaking (100 RPM).
Search related documents:
Co phrase search for related documents- Cell culture and dna plasmid: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18
- Cell culture and room temperature: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35
- culture volume and room temperature: 1, 2, 3, 4, 5, 6
- dna plasmid and room temperature: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12
- dna plasmid and transfection mix: 1
- dna plasmid ml and room temperature: 1
- dna plasmid ml and transfection mix: 1
- room temperature and transfection mix: 1
Co phrase search for related documents, hyperlinks ordered by date