Author: Bouvette, Jonathan; Korkut, Dursun Nizam; Fouillen, Aurélien; Amellah, Soumiya; Nanci, Antonio; Durocher, Yves; Omichinski, James G.; Legault, Pascale
Title: High-yield production of human Dicer by transfection of human HEK293-EBNA1 cells grown in suspension Document date: 2018_12_6
ID: 012ipcdr_31
Snippet: Cell cultures were diluted to 0.8 × 10 6 cells/mL 24 h before transfection. The transfection mix was prepared in 10% of the final culture volume in F17 medium with 10 μg/mL of DNA plasmid and 20 μg/mL of linear PEI 25 kDa (Polysciences Inc.). The mix was agitated by 3 vortexing cycles of 3 s, incubated 15 min at room temperature and incorporated in the culture [32] , which was incubated for 72-96 h (37°C, 5% CO 2 ) with shaking (100 RPM)......
Document: Cell cultures were diluted to 0.8 × 10 6 cells/mL 24 h before transfection. The transfection mix was prepared in 10% of the final culture volume in F17 medium with 10 μg/mL of DNA plasmid and 20 μg/mL of linear PEI 25 kDa (Polysciences Inc.). The mix was agitated by 3 vortexing cycles of 3 s, incubated 15 min at room temperature and incorporated in the culture [32] , which was incubated for 72-96 h (37°C, 5% CO 2 ) with shaking (100 RPM).
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