Author: Schildgen, Oliver
Title: Human Bocavirus: Lessons Learned to Date Document date: 2013_1_11
ID: 1qyuhbz0_5
Snippet: The HBoV genome length ranges from 5,217 to 5,299 nucleotides [4] plus the terminal sequences, which are between 32 and 52 bases [7, 8] . Based on phylogenetic analyses and electron microscopy analyses of the HBoV genomes from clinical specimens, the genome is believed to consist of a single-stranded DNA [9, 10] of negative polarity. The negative polarity has been confirmed by real-time NASBA [11] . The terminal sequences, which are assumed to pl.....
Document: The HBoV genome length ranges from 5,217 to 5,299 nucleotides [4] plus the terminal sequences, which are between 32 and 52 bases [7, 8] . Based on phylogenetic analyses and electron microscopy analyses of the HBoV genomes from clinical specimens, the genome is believed to consist of a single-stranded DNA [9, 10] of negative polarity. The negative polarity has been confirmed by real-time NASBA [11] . The terminal sequences, which are assumed to play a crucial role in the initiation of viral replication in the Parvoviridae family, have been identified as linker sequences in episomal genomes or replication intermediates in HBoV1 [7] and HBoV3 [8] and have been shown to have high homology to the terminal sequences of minute virus of canines (MVC) and bovine parvovirus [4] , the latter providing a hint about the zoonotic origin of HBoV, either decades or centuries ago.
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