Author: Pattyn, Els; Verhee, Annick; Uyttendaele, Isabel; Piessevaux, Julie; Timmerman, Evy; Gevaert, Kris; Vandekerckhove, Joël; Peelman, Frank; Tavernier, Jan
Title: HyperISGylation of Old World Monkey ISG15 in Human Cells Document date: 2008_6_18
ID: 1eksm537_43
Snippet: The concentration of the protein was determined by the Bradford assay. 30 mL was loaded on a 12% SDS page gel and silver stained to check the quality and purity of the ISG15 proteins. Purified recombinant UbE1, UbE1L, UbcH8, UbcH10 and UbcH13 were purchased (Boston Biochem)......
Document: The concentration of the protein was determined by the Bradford assay. 30 mL was loaded on a 12% SDS page gel and silver stained to check the quality and purity of the ISG15 proteins. Purified recombinant UbE1, UbE1L, UbcH8, UbcH10 and UbcH13 were purchased (Boston Biochem).
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