Author: Kummer, Susann; Avinoam, Ori; Kräusslich, Hans-Georg
Title: IFITM3 Clusters on Virus Containing Endosomes and Lysosomes Early in the Influenza A Infection of Human Airway Epithelial Cells Document date: 2019_6_12
ID: 1345qct4_20
Snippet: To determine whether an IAV-induced viral membrane fusion and genome uncoating are required for the observed IFITM3 signal increase upon IAV infection, we performed experiments in the presence of Bafilomycin A1, specifically inhibiting endosomal acidification, or in the presence of Amantadine, specifically blocking the tetrameric M2 channel of IAV, thereby preventing genome uncoating. In both cases, no increase in the IFITM3 signal intensity and .....
Document: To determine whether an IAV-induced viral membrane fusion and genome uncoating are required for the observed IFITM3 signal increase upon IAV infection, we performed experiments in the presence of Bafilomycin A1, specifically inhibiting endosomal acidification, or in the presence of Amantadine, specifically blocking the tetrameric M2 channel of IAV, thereby preventing genome uncoating. In both cases, no increase in the IFITM3 signal intensity and no IFITM3 clustering were observed ( Figure 1D ,E). These results indicate that IAV-induced membrane fusion and genome uncoating are required for an IFITM3 signal increase in A549 cells.
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