Author: Rhein, Bethany A.; Powers, Linda S.; Rogers, Kai; Anantpadma, Manu; Singh, Brajesh K.; Sakurai, Yasuteru; Bair, Thomas; Miller-Hunt, Catherine; Sinn, Patrick; Davey, Robert A.; Monick, Martha M.; Maury, Wendy
Title: Interferon-? Inhibits Ebola Virus Infection Document date: 2015_11_12
ID: 10bu7iwg_64
Snippet: ISG inhibition of infectious EBOV virus was performed using Neon (Invitrogen) electroporated HeLa cells (Ambion, Austin, TX). Forty-eight hours following transfection, cells were infected with EBOV-GFP at MOI of 0.5. After 24 hours, infection was quantified as described previously [25] . Briefly, images were analyzed using Cell Profiler to quantify RFP and GFP positive cells. Data were analyzed with FCS Express analysis software and cells were ga.....
Document: ISG inhibition of infectious EBOV virus was performed using Neon (Invitrogen) electroporated HeLa cells (Ambion, Austin, TX). Forty-eight hours following transfection, cells were infected with EBOV-GFP at MOI of 0.5. After 24 hours, infection was quantified as described previously [25] . Briefly, images were analyzed using Cell Profiler to quantify RFP and GFP positive cells. Data were analyzed with FCS Express analysis software and cells were gated based on the no infection control.
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